Asia-Pacific Dermatology and Cosmetology Conference August 16-17, 2018 Tokyo, Japan

Toni Febriyanto is a medical student of Universitas Gadjah Mada, Yogyakarta, Indonesia. He is interested in biomedical and clinical research

Background: Ultraviolet (UV) exposure causes photoaging by inducing the expression of matrix metalloproteinase-1 (MMP-1) expression. Snail Achatina fulica is one of the most extensively studied snails due to its economic, ecological and medical importance. Moreover, the slime is well-known for its wound-healing and anti-bacterial properties. Objective: The research is aimed to investigate whether snail slime of Achatina fulica exhibits anti-photoaging properties by inhibiting the expression of MMP-1. Method: Fifty snails were stimulated by a 9V electricity source for the extraction of slime. The extracted slime was then converted into powder form by the freeze-drying process. The slime powder was dissolved in dimethyl sulfoxide (DMSO) to desired concentrations. At the end of the experiment, the MMP-1 expression in different fibroblast groups was assessed by qPCR. Results: Administration of snail slime has proven to lower MMP-1 expression compared to the positive control group without snail slime. However, administration of snail slime at various concentrations did not significantly produce different results. MMP-1 expression level is slightly lower in slime group compared to the Ellicina cream group at the concentration of 25 and 50 µg/mL. Administration of Ellicina cream at the concentration of 100 µg/mL has shown to lower MMP-1 expression compared to slime group at any concentration. Conclusions: Snail slime of Achatina fulica reduces MMP-1 expression level on UVB-induced fibroblast culture. The potency in inhibiting MMP-1 expression level between Achatina fulica slime and Ellicina cream is not much different at certain concentrations.

Lecturer in Dept of Dermatology at Kathmandu medical college teaching hospital,Nepal

Acne is a multifactorial disease ranging from a couple of comedones and pustules to severe nodulocystic fulminant acne. Isotretinoin(13-cis-retinoic-acid) is the only drug that can affect all the factors involved in the pathogenesis of acne and approved by FDA in 1982 and cause several dose-dependent mucocutaneous and systemic side effects and cost-effectiveness. Objectives: To assess and compare the efficacy and tolerability of two regimens ( daily conventional, pulse dose ) in moderate to severe acne vulgaris. Method: This is a prospective randomized comparative study in moderate to severe acne vulgaris in Kathmandu Medical College Teaching Hospital. The patients were randomly divided into two groups (A and B). In a group, A conventional dose of 20 mg of oral isotretinoin was given daily and in the group, B 20 mg of oral isotretinoin was given thrice (Pulse Dosage in the alternate day ) in a week for 6months. The baseline hematological, biochemical and radiological (lumbosacral spine)was compared on first, third and six month. Result: 74 patients were enrolled with a mean age of 21.73 years. Patients with daily regimen show good response throughout the therapy for six months (1st, 3rd 6th months), whereas those in pulse therapy show moderate to poor response during the early month and gradually improve by end of 6th month. At end of 6 months, both the regimen show a good response and was statistically significant. Conclusion: However, the pulse dose regimen makes the treatment more acceptable for patients with than the classical standard and daily regimen, in term of side effects and cost effectiveness and patient compliance.